Antimicrobial and Antioxidant Evaluation of Bioactive Purified from Medicinal Plant Against Klebsiella Pneumoniae

Abstract

newline ABSTRACT newlinePlants are a rich source of therapeutic compounds that are widely used in the newlinepharmaceutical industry. Methanolic and aqueous (water) extracts of five medicinal newlineplants were systematically evaluated to find new sources of antimicrobial and newlineantioxidant substances. In addition, phytochemical screening was performed using newlinestandard qualitative tests to identify various secondary metabolites. Natural newlineantioxidants were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical newlinescavenging process. Methanolic and aqueous (water) extracts were prepared from the newlineplant materials and their antibacterial activity was evaluated against standard bacterial newlinestrains representing both Gram-positive and Gram-negative types. To evaluate the newlineantibacterial activity of the extracts, the minimum inhibitory concentration (MIC) was newlinedetermined using an enzyme-linked immunosorbent assay (ELISA) to read the newlineinhibition. These five plants had a broad spectrum of antibacterial activity. The results newlineshowed the antibacterial efficiency of the methanolic extracts of A. indica, A. cepa, E. newlineglobulus, N. indicum and Z. officinale inhibited bacterial strains with different MIC newlinevalues from 2.443 mg/ml to 642.6 mg/ml. Therefore, in light of these results, the newlineability of the extracts to inhibit the growth of several bacterial species indicates the newlinepresence of broad-spectrum antimicrobial potential, making the plants a candidate for newlinebiological discovery of antimicrobial drugs. Antibiofilm activities were carried out by newlineK. pneumoniae and S. pneumonia against two medicinal extracts A. indica and Z. newlineofficinale. Plant extracts mode of action provided evidence for the disintegration of newlinebacterial strains. The most potent inhibitory effect of A. indica against K. pneumoniae newlinewas observed. The results of the phytochemical screening indicated the presence of newlinealkaloids, flavonoids, cardiac glycosides, tannins, saponins, steroids, and phenols. The newlineresearch identifies plants with antimicrobial and antioxidant properties that could be newlinexi