Proteomic analysis of renal disorders and LDH GOX inhibition studies with reference to Urolithiasis

Abstract

Urolithiasis (urinary tract calculi or stones) is a well-documented renal abnormality in the general population of world. The etiology of this disorder is multifactorial and is strongly related to dietary lifestyle habits or practices. Proper management of calculi that occur along the urinary tract includes investigation into causative factors in an efforts to prevent recurrences. newlineIn this study. Wistar rat enzyme preparations were utilized for in vitro assay of enzymes. Glycolate oxidase (GOX) and lactate dehydrogenase (LDH), known to play a key role in oxalate synthesis as that of human enzymes. In present study indicated that glycolate oxidase and lactate dehydrogenase were extracted from rat liver, partially purified by ion exchange chromatography (DEAE-Cellulose -52) method and characterized with SDS-PAGE and Iso-Electric Focusing. Subsequently, screened Saraca asoca, Bergenia ciliata, Crataeva nurvala and Boerhavia diffusa plants exhibited inhibition against different biochemical parameters involved in etiology of CaOx urolith formation like enzymes GOX and LDH, CaOx aggregation and nucleation in a dose dependent manner and furthermore antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl free radical. newlineIso-Electric Focusing and Sodium Dodecyl Sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) study revealed enzyme glycolate oxidase with PI value 9 with Molecular weight ~41 KDa and enzyme lactate dehydrogenase with molecular weight ~35 KDa. Ethanolic extracts of Saraca asoca, Bergenia ciliata, Crataeva nurvala and Boerhavia diffusa were characterized via UV-FTIR and GC-MS which revealed presence of alcohols, phenols, alkanes, alkynes, alkyl halides, aldehydes, carboxylic acids, aromatics, nitro compounds and amines. All twenty three phytocompounds were analysed for PASS to check the probability of a compound to be active or inactive and the analysis revealed compounds activity was PagtPi along with the glycolate oxidase and lactate dehydrogenase inhibitory potential with different values. Further, compo