Synthesis Of Silver Nano Particles By Bacterial Isolate E Coli Spp And Their Antibacterial Activity

Abstract

newline ABSTRACT newlineThe use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach. In the present study, we observed the synthesis of silver nanoparticles and reported the biosynthesis of silver nanoparticles employing the bacterium E.coli. The multi drug resistant pathogen E.coli was chosen due to its highest AgNP synthesis. The higher level of nitrate reductase activity was measured at 731.24 U/ml with presence of glucose: 0.1%, peptone: 1%, yeast extract: 0.4%, KNO3: 0.4%, pH: 7.5 at 25 and#730;C and 3 days incubation period. The spectrophotometric analysis of AgNPs at the band and#411; 431 nm for characteristic Surface Plasmon Resonance which indicated that the Ag nanoparticles are spherical or roughly spherical shaped. FT-IR spectrum of AgNPs the bands observed at 1359.35 cm 1, 1350.3 cm-1 and 1398.40cm-1. Increasing concentration of AgNO3 showed more effectiveness when treated with gamma radiation after mixing than before mixing with silver nitrate solution. The AgNPs synthesis showed the antimicrobial activity for the E.coli measured was 18.67±2.082 diameter in zone on inhibition. The AgNPs synthesized values of MIC against the tested clinical pathogen showed the antimicrobial activity at 200 ppm for E.coli. newline newlineKeywords: Microbial production of Silver nanoparticles; E. coli bacteria; UV-Vis spectroscopy; Transmission Electron Microscopy; Antibacterial activity; Bacterial growth curve. newline newline