Genetic diversity of high level Gentamicin resistance in clinical Isolates of enterococcus faecalis

Abstract

newlineEnterococcus faecalis is gaining importance in the nosocomial newlineenvironment due to the development of antibiotic resistance The commonly newlineused method of control against E faecalis is a synergistic combination of a newlineglycopeptide along with an aminoglycoside The most commonly used newlineaminoglycoside is gentamicin High level gentamicin resistance HLGR newline MIC 500mg ml is mainly conferred by the fused gene aac 6 Ie aph 2 newlineIa encoding the aminoglycoside modifying enzyme aminoglycoside newlineacetyltransferase and phosphotransferase This gene is present in plasmids newlineand or chromosomes associated with transposable elements which favour newlinehorizontal transfer of HLGR The molecular basis of HLGR in clinical newlineisolates prevalent in India has not yet been analysed newlineThis study was aimed to determine the genetic factors conferring newlineHLGR their location and the frequency of transfer using 40 clinical isolates newlineof E faecalis newline newline newline