Dissipation Kinetics of Certain Pesticides in Blood Identification of Residues in Biological and Environmental Samples

Abstract

In an exposure study, analysis of blood for residue estimation is a difficult task. In general, the residues in blood are likely to appear at very low concentrations, because as soon as it enters in to the body most of the chemical may get excreted. Nevertheless, the ultra low quantities present if any, are enough to create significant impact on human health. This prompted to the present investigations and developed different extraction procedures and sensitive analytical methods capable of detecting the residues at trace levels. In the category of agrochemicals, presence of trace quantities of pesticides directly influences the human health due to their carcinogenic and mutagenic factors. The only way to monitor the pesticide residue is to investigate their presence or absence using a selective and sensitive analytical method. Present investigations are aimed at developing different analytical techniques capable of detecting the residues of different class of pesticides in biological and environmental samples and to investigate the dissipation kinetics in whole blood. Research work presented in the thesis mainly emphasizes pyrethroid insecticides, chlorinated insecticides,1,3,5-triazine herbicides and chlorinated herbicides. New hi-phonated techniques such as negative ion chemical ionization gas chromatography mass spectrometry method, electron ionization gas chromatography mass spectrometry method are used. Apart from this solid phase micro extraction techniques is used to detect the residues at very low levels. The details of investigations conducted on 36 pesticides are as follows. For the quantification of 13 synthetic pyrethroid insecticide molecules namely allethrin, bifenthrin, cypermethrin, cyphenothrin, cyfluthrin, lambdacyhalothrin, deltamethrin, fenvalerate, fenpropathrin, imiprothrin, permethrin, prallethrin, transfluthrin and their stereo isomers in whole blood A new rapid and sensitive electron ionization gas chromatography-mass spectrometry method in selective ion monitoring mode (SIM) has been developed.