An approach to enhancement of dye degrading activity of laccase produced by solid state fermentation through its immobilization on chitosan coated magnetic nanoparticles

Abstract

Nanotechnology is an emerging field of science which provides an understanding of the control of matter at the nanoscale level newlineNanoparticlesare rich in surface composition and properties which are useful newlinefor functional applications including catalysis Laccases a kind of oxidative newlineenzymes are of considerable interest in industrial and environmental newlinebiotechnology Laccases isolated from different fungal sources have so far newlinebeen used for the degradation of a wide variety of dyes Hence the choice of newlinefungus in this study for laccase production was Pleurotus ostreatus Musa newlinecavendish Peels MCP was taken as the novel natural substrate since the newlinefungi reproduce more effectively under the natural environmental conditions newlinePlankett Burman design PBD was performed to screen the newlinesignificant factors affecting laccase production from MCP using Pleurotuso newlinestreatus among selected five factors And further optimization using Central newlineComposite Design CCD revealed optimum values of pH 6 Temperature 25C and substrate to moisture ratio of 12 with maximum laccase activity of 446 Umg newline newline