Evaluate the Efficacy of Real Time PCR for MBL Detection in Phenotypically Confirmed Clinical Isolates of Pseudomonas Aeruginosa in a Tertiary Care Hospital

Abstract

newline ABSTRACT newlineINTRODUCTION: Pseudomonas aeruginosa is a significant isolate from gram-negative bacterial infections in humans worldwide, despite upgrades in antibiotic treatment. P. aeruginosa is intrinsically resistant to several antimicrobial agents. It is ubiquitous and mainly present as a saprophyte in warm moist conditions in the aqueous and environmental sources, including sinks, drains, respirators, humidifiers, and disinfectant solutions. newlineMetallo and#946;-lactamase gene was integron-mediated resistance, first isolated among Enterobacteriaceae, Pseudomonas, and other non-fastidious gram-negative bacilli isolated in several hospitals in Japan (10). These isolates are responsible for severe infections such as septicemia and pneumonia and accountable for therapy failure with carbapenems. Recently, resistance to carbapenem among members of Enterobacteriaceae and non-fermentative gram-negative rods has become a primary health-related concern worldwide. newlineAIM and OBJECTIVES: To evaluate the efficacy of real-time PCR for MBL detection in phenotypically confirmed isolates of P. aeruginosa in various clinical samples. To study the antibiotic susceptibility pattern of Pseudomonas aeruginosa in various clinical specimens. To compare detection of Metallo Beta-Lactamase (MBL) in Pseudomonas aeruginosa by different phenotypic methods. To isolate and identify the prevalence of MBL in carbapenem-resistant Pseudomonas aeruginosa in various clinical samples. To determine the minimum inhibition concentration (MIC) by E-test. To correlate the results of the genotypic method with phenotypic methods and to perform real-time PCR in phenotypically confirmed MBL-producing strains of P. aeruginosa and correlate the results of the genotypic method with phenotypic detection. newlineMATERIALS AND METHODS: A total of 200 Pseudomonas aeruginosa were isolated from different clinical samples collected from outpatients, and inpatients were tested for the newlinepresence of metallo-beta-lactamase. Susceptibility testing for carbapenems was performed by the d