Comparative Analysis of Vitexin and Quercetin Present in the Leaves of Vitex negundo Linn from Himachal Pradesh

Abstract

newline COMPARATIVE ANALYSIS OF VITEXIN AND QUERCETIN PRESENT IN THE LEAVES OF VITEX NEGUNDO LINN. newlineFROM HIMACHAL PRADESH newline newlineFaculty of Biotechnology newlineShoolini University of Biotechnology and Management Sciences newlineSolan, Himachal Pradesh, India newline newline newlineABSTRACT newline Vitex negundo is a large aromatic shrub, having yellowish brown bark, has 3-5 foliolate leaves, with bluish-white flowers. It is commonly known as Nirgundi, Sambhalu, Banna etc. and is found throughout India upto an altitude of 2200 m. Preparations from V. negundo plant parts are commercially used in various ayurvedic medicines and ointments to cure various diseases such as rheumatic disorders, arthritis, gout, cervical spondylitis, inflammatory musculoskeletal disorders, haemorrhoids (Piles), rheumatic pain, sprain, tooth ache, wounds, burns and fungal skin infection. Although V. negundo is abundant in Himachal Pradesh very less efforts have been made to exploit the therapeutic potential of secondary metabolites from V. negundo such as Vitexin and Quercetin which have various beneficial properties that can be harnessed for treatment and prevention of numerous human diseases. Vitexin and Quercetin share some major therapeutic properties such as they both are apoptosis inducer, tumor suppressor, cardioprotective, anti-inflammatory, neuroprotective and have antioxidant properties. Keeping in mind the potential in drug formulation Vitexin and Quercetin were chosen for this study. newline newline Samples of Vitex negundo leaves were collected in the month of April from 120 sites spanning within 8 districts of Himachal Pradesh. Methanolic leaf extracts of Vitex negundo were prepared by successive extraction using Soxhlet apparatus and were subjected to RP-HPLC for quantification of Vitexin and Quercetin. In the present study we found that the concentration of Vitexin and Quercetin in leaves of Vitex negundo did not have any significant difference among the collected samples from Himachal Pradesh. Concentration of vitexin was almost constant i.e. 2.7955 µg/mL whil