Design Development and Characterization of Biosensors and Nanoparticulate Carriers for Renal Cell Carcinoma

Abstract

Magnetic-core-based silibinin nanopolymeric carriers for the treatment of renal cell cancer.The synthesized iron oxide nanoparticles were prepared by precipitation method via encapsulation of silibinin in PLGA network using double emulsion method. The nanoparticle formulations were characterized for morphological, physicochemical properties (HRTEM, FTIR, Raman Spectroscopy and VSM), in-vitro drug release and cytotoxicity study on kidney cancer cells (A-498). The safety of magnetic-core-based silibinin nanopolymeric carriers was conducted byi.v. administration at a dose of 50 mg/kg in mice. newlineMagnetic-core-based silibinin nanopolymeric carriers were prepared with the double emulsion solvent evaporation method for the treatment of renal cell cancer. The newly developed drug delivery system possessed an acceptable particle size, zeta potential and high % EE with a greater inhibitory effect on the growth of kidney cancer cells (A-498)as compared to free silibinin. Stability studies indicated that the storage of SLB-MPNPs at refrigerated conditions increases the stability of the formulation for a period of 3 months. Acute toxicity study revealed that SLB-MPNPs were found to be safe at a dose of 50 mg/kg for administration in mice. Silibinin is a clinically accepted herbal drug with minimum side effects, therefore the magnetic-core-based silibinin nanopolymeric carriers considered as an effective targeted drug delivery system for treating renal cell cancer. newlineMonodispersed magnetographene quantum dot nanocomposites for delivery of silibinin newlineSilibinin shows multi-applications and therapeutic activities such as anticancer, antioxidant, hypocholesterolemic, cardioprotective, neuroprotective and hepatoprotective but exhibits limited clinical applications due to poor oral bioavailability and in-vitro in-vivo biological correlation. The present study focused on the characterization of magnetic graphene quantum dot nanocomposites for silibinin delivery with a specific interest in kidney cancer treatment..