Formulation and Evaluation of Novel In Situ Gel Forming System for Nasal Delivery of Drug

Abstract

Migraine is a neurological disease and disorders of the systema nervosum that can cause throbbing, pulsing headache on one side of your head. Regular uses of medicine either by oral or IV route precipitate serious side effect such as heart attack, stroke, kidney damage, stomach ulcers and it is due to unwanted distribution of drug. In the present work, it was attempt to develop such a strategy that can deliver drug direct to the brain through nasal route. The almotriptan loaded ethosomes containing nano in-situ gel system was prepared that can release drug in controlled manner and transport from nostril to brain directly via olfactory region. The formulation will remain in solution form and it will become be gel for upon interaction with the physiological condition like pH, ions and temperature of nasal cavity. Almotriptan causes extracerebral, intracranial blood vessel constriction via binding to serotonin 5-HT 1B and 1D receptors in the central nervous system (CNS) that reduce extravasation of plasma proteins, and lowering the release of other inflammatory mediators from the trigeminal nerve which help to relief the headaches.. The nano insitu gel was further characterized for drug content, viscosity, gel strength, gel capacity, gelling time, spreadability and in-vitro drug release study. The prepared gel formulation was found 8.5 cps viscosity of optimized formulation in the form of sol which was optimum for nasal administration. The prepared optimized formulation was shown significant gelling capacity and strength and mucoadhesivness which retain the drug in nasal cavity to release, absorb and transport in therapeutic amount to the brain.. In-vitro drug release was carried out in acetate buffer pH 6.4 using French diffusion cell . It was found that the formulation was non- irritant to the nasal mucosa which was confirmed by performing histopathology study. Drug distribution was revealed that the drug was found higher in concentration in the brain when it compare with normal drug solution. The prepared optimized nano in-situ nasal gel formulation will capable to deliver drug to the brain in higher amount with minimum side effect with low cost of treatment. newline