Insilico Identification and Expression Analysis of miRNA564 and its Target Proteins in Gastric Carcinoma

Abstract

Gastric cancer is the major health problem in both men and female No efficient biomarker newlineis available to characterize the mechanism of the cancer especially with regards to newlinemetastasis Searching for potential gene is a key to understand the molecular mechanism newlinebehind the gastric cancer development In this study I applied a comprehensive strategy newline to identify miRNA by searching human EST sequences involved in gastric cancer newlineagainst known mature miRNA sequences and to predict its target protein I also identified newline the expression of miRNA by Real Time PCR and target protein expression newline by immunohistochemical technique in both gastric cancer and adjacent normal tissues newlineThe expression of miR564 was down regulated in gastric cancer tissues compared newlineto the adjacent normal gastric tissues Target protein RelA and IL6R expressions newlinesignificantly increased in gastric cancer tissues The expression of both miRNA newlineand targets proteins were negatively correlated which indicated that miR564 might newlinebe a novel negative regulator of RelA and IL6R proteins and plays a vital role in newlinetumorigenesis of gastric cancer The expression of miR564 was significantly correlated newline with the depth of tumour invasion suggest that miR-654 may be involved in the progression newline of gastric cancer This data reveals that miR-564 acts as a potential tumour suppressor and newline miR564 RelA IL6R complex may be a novel prognostic and therapeutic target for newlinegastric cancer treatment newline newline