Evaluation And Molecular Characterization Of leaf And Stripe Rust Of Wheat

Abstract

newline Leaf rust and Stripe rust of wheat are responsible for significant losses in yield potential.Although, chemical control of thesediseasesisknownbutitisnoteconomicandenvironmentally friendly.Inpresentinvestigation wasduetofrequent breakdown of major seedling resistance genes in recent years and difficulties in quick replacement of susceptible wheat varietiesleadstoinvestigatesofotherformofdiseaseresistancelikeadultplantresistance(APR)andslowrustingwhich are considered asmore durable. Out of 96 germplasm and three susceptible checks viz.,Agra local, HD2967, PBW343 for stripe rust,Agra local, Karachia-65and Lalbahadur for leaf rust, nine germplasm GWJ39, GWJ48, GWJ50, GWJ53, GWJ55, GWJ64, GWJ66, GWJ77 and GJW83 for stripe rust, ten germplasm viz., GWJ38, GWJ39, GWJ56, GWJ57, GWJ61,GWJ75,GWJ77,GWJ88,GWJ89andGWJ90forleafrustshowedimmuneresponsebothatseedlingstageand adultplantstage.Seventeengermplasmviz.,GWJ5,GWJ6,GWJ10,GWJ18,GWJ19,GWJ34,GWJ35,GWJ36,GWJ37, GWJ38, GWJ45, GWJ52, GWJ54, GWJ59 GWJ69, GWJ89 and GWJ91 showed resistant reaction in seedling stage against stripe rust pathotypes but these lines become susceptible at adult plant stage. Eleven germplasm viz., GWJ3, GWJ6,GWJ33,GWJ51,GWJ54,GWJ55,GWJ69,GWJ76,GWJ81,GWJ83,GWJ84,showedseedlingresistant newlinereaction against leaf rust pathotypes but these lines become susceptible at adult plant stage. Forty-one germplasm were observedwithACIvaluebetweengt0to10asresistantatadultplantstagetostriperust,fifty-sixgermplasmwiththeACI value ranges between gt0 to 10 to leaf rust.ACI values between gt10 to 20 was recorded in fifteen germplasm for stripe rust and 23 germplasmfor leaf rust consideredasslowrustier line.Molecularcharacterization ofeight Yrgenelike Yr2, Yr5, Yr9, Yr10,Yr15, Yr16, Yr18 and Yr29andthreeLr genelike Lr32, Lr50, Lr67. Yr5 withACI valuein between 0to 5 in thirteen germplasm with frequency of 13.5%. Yr10 race specific withACI range between 0-5 postulated in twelve germplasm with frequency of 12.5 showed resistance response. Yr15 withACI range between 0