Study of Anti Neoplastic Potential In Green Synthesized Silver Nanoparticals on Selected Hunman Cancer Cell Lines

Abstract

iv newlineABSTRACT newlineThe green synthesis of metal nanoparticle is a booming research area in the current newlineNano-science and technology. Exploitation of the silver nanoparticles in the field of newlineNano medicines is increasing owing to its positive effects, such as, antimicrobial, newlineanticancer and antioxidant activities. Though nanoparticles are synthesized through newlinephysical and chemical approaches effectively, they are not so eco-friendly. The rationale newlineof the present study is to develop a fast, more eco-friendly, and green synthesis for silver newlinenanoparticle and to study its anticancer effect on different cancer cell-lines for the bio- newlinemedical applications. The objective of the study is to synthesize silver nanoparticles from newlineleaf extract of Withania somnifera, Aegle marmelos, Taraxacum officinale and to show newlineits cytotoxic effects on selected human cancer cell-lines. Green synthesis of silver newlinenanoparticles are characterized by using UV- Vis spectroscopy, Fourier Transform newlineInfrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Energy newlineDispersive X-ray spectroscopy (EDX). Synthesized silver nanoparticles were of irregular newlineshape and the estimated sizes were 18nm, 94nm and 26nm respectively as characterized newlineusing SEM. Further characterization, which was done using EDX, UV-vis newlinespectrophotometer and FTIR also confirmed the formation of silver nanoparticles. Silver newlinenanoparticles synthesized from Withania somnifera, Aegle marmelos and Taraxacum newlineofficinale by MTT assay at various concentrations showed the cytotoxic activity newlineeffectively. DNA Fragmentation by TUNEL Assay is performed for the assessment of newlineapoptosis. Silver nanoparticles which are efficiently synthesized using the leaves of newlineWithania somnifera, Aegle marmelos, Taraxacum officinale, can be used in the bio- newlinemedical applications owing to its cytotoxic activity against cancer cell lines. newlineKey Words: Green synthesis of silver nanoparticles using Withania somnifera, Aegle newlinemarmelos and Taraxacum officinale, Characterization of silver nanoparticles-UV- newlineVis, FTIR, SEM