Bioprospection of Bistorta amplexicaulis D Don Greene A valuable medicinal plant growing in Kashmir Himalaya

Abstract

The utilization of plant resources for medicinal purposes dates back to the dawn of civilization, with over 80,000 species identified for their therapeutic value. Traditional systems of medicine have extensively utilized these plants, which remain a primary healthcare resource for 80% of the global population according to the World Health Organization. India, with its rich biodiversity, particularly in regions like the Kashmir Himalaya, harbours numerous medicinal plants facing threats from over-exploitation, habitat loss, and climate change. Bistorta amplexicaulis (D. Don) Greene, a significant medicinal plant from this region, is under threat due to these pressures. This study focuses on the micropropagation and conservation of B. amplexicaulis, aiming to develop efficient in vitro propagation protocols. These methods include the use of plant tissue culture techniques for the large-scale production and conservation of this species, along with the elicitation of secondary metabolites. The research emphasizes the importance of biotechnological approaches in producing bioactive compounds, thereby reducing pressure on wild populations. Phytochemical analysis, including Gas Chromatography-Mass Spectroscopy (GC-MS), was conducted to identify active compounds and assess their medicinal potential; particularly their anticancerous activity. The findings reveal that in vitro propagation techniques can significantly enhance the production of medicinally valuable secondary metabolites. This study contributes to the conservation of B. amplexicaulis and validates its sustainable use in traditional and modern medicine systems. The successful development of in vitro propagation protocols and phytochemical analysis of in vitro and in vivo plant parts underline the potential of tissue culture techniques in preserving and utilizing medicinal plants effectively. newlinePlant biotechnology is utilized for the conservation and sustainable use of medicinal and aromatic plants (MAPs), which are critical for healthcare, especially in ...