Detection of resistance nodulation division efflux pump AdeABC in tigecycline resistant Acinetobacter baumannii

Abstract

The development of novel efflux pump inhibitors and other alternative newlinemedicinal medications require an understanding of the contribution, function, newlineand regulation of multidrug efflux systems. While A. baumannii has long been newlinerecognized as a clinically significant pathogen, not much is known about its newlinemultidrug efflux systems, particularly in clinical isolates. Hence there is a need newlineto determine the role of the multidrug efflux system in the resistance of newlineantibiotics in A. baumannii clinical isolates, so that alternative strategies for newlinetreating infections caused by this bacteria can be developed. newlineAim newlineTo study the role of AdeABC efflux pump that causes resistance to last newlineresort antibiotic tigecycline from multidrug-resistant Acinetobacter baumannii newlineclinical isolates. newlineThe present study newlinealso evaluated the role of adeB gene in causing resistance to various newlineantibiotics by docking studies where the energy binding efficacy of various newlineantibiotics showed the role of adeB gene in extruding these antibiotics from newlinethe bacterial cell. And also the natural compound usnic acid has been newlineevaluated in docking study to see its efficacy as efflux pump inhibitor to adeB newlinegene of AdeABC efflux pump and found to be a good inhibitor. The newlineimportance of testing resistant isolates for the presence of efflux pumps, to newlineidentify a number of antibiotics. Phenotypic and genotypic identification of AB newlineinfections should be performed to ensure appropriate treatment. There is a newlineneed for research in the detection of efflux pump resistance and identify new newlineefflux pump inhibitors that can be used along with antibiotics as adjuvants in newlinetreating the MDR A. baumannii infections newline