Evaluation of Various Methods for the Detection of Biofilm Formation by Uropathogenic Escherichia Coli in Tropical Catheterised Patients

Abstract

The Present study aimed to investigate the efficacy of different methods of biofilm detection in Tropical catheterized patients. The objectives of the present study included comparison of efficacy of Tissue Culture Plate Method, Modified Congo Red Agar Method and Tube Adherence Method for detection of biofilm formation by multidrug resistant Uropathogenic Escherichia coli in Tropical catheterized patients. The study also investigated the analytical efficacy of Modified Congo Red Agar method and Tube Adherence Method against Tissue Culture Plate method followed by evaluation of correlation of the biofilm formation by Multi Drug Resistant E. Coli with age, sex and Risk Factors of the Tropical catheterized patients. The study included a total of 500 urine specimens from patients admitted with UTI. In this study different microorganisms were isolated from the urine samples collected from 500 patients and spotted as potential biofilm producer following evaluating their antibiogram biofilm production was tested of the concerned microorganisms. . As per the study, female patients were found be the major host of biofilm producers as compared to male counterpart as suggested by the statistical analysis. Demographically rural patients in the present study were observed to be more susceptible to biofilm producing microorganisms than the urban patients. The antibiotic susceptibility study indicated that carbapenams, polymyxins and tetracyclins, newlineTigecycline, Polymyxin B and Colistin were the effective drugs against biofllm producers. However, the results indicated that there is no statistical association between diabetes as factor responsible and the type of microscopic isolate in the study. newline newline