Genome wide survey of certain mammalian gpcrs and olfactory receptors

Abstract

In the recent era of Gprotein coupled receptor GPCR research newlinecomputational approaches in sequence analysis play a vital role in identifying newlinerelated sequences homologues conserved features domains motifs and newlineevolutionary impacts orthologs for the interested protein families at intraand newlineintergenomic levels Candidate GPCRs and ORs class A type GPCR newlineare important for their diverse cellular activities and have been considered for newlinethe genomewide survey in selected eukaryotic genomes which further helps newlineto establish a structure function resemblance newlineGenerally GPCRs are predicted for having extracellular Nterminal newlineNout topology intracellular Cterminal with seven transmembranehelices newlineTMHs and are connected by three intra and extracellular loops thereby newlinetermed as serpentinelike receptors newlinePrevious crossgenome studies on human Drosophila GPCRs newlinemotivated to perform a crossgenome clustering on human C elegans newlineGPCRs Chapter 2 A profile based clustering RPSBLAST was employed newlineto associate more than 1000 C elegans GPCRs with already grouped human newlineGPCR clusters of eight major types of receptors The generated 32 human C newlineelegans GPCR clusters were analyzed for five different types of cluster newlineassociation with proposed terminologies such as human GPCR clade HC newlinecoclusters CC neighbor clades NC neighbor members NM speciesspecific newlinemembers SS observed at tree topology which facilitate to connect newlinefunctional relevance at intraand intergenomic levels Interestingly the referred newlineCC was significant and exhibited evolutionary integrity at intergenomic level newlineAlso the identified 27 orthologs were evident to illustrate the effectiveness of newlineusing crossgenome clustering techniques in connecting related GPCRs even at newlineremote homology Overall 84 of the GPCR sequences across genomes have newlinebeen associated at the significant Evalue thresholds ranges from 0001 to 1 newlinesuccessfully by RPSBLAST work published newlineCrossgenome clustering on human and C elegans GPCRs motivated newlineto perform a phylogenetic analysis on serpentine receptors SRs exclusively newlineChapter 3 newline