Gross Anatomical Histological and Histochemical Studies on the Intestine of Prenatal Goat Capra hircus

Abstract

On perusal of literature it has been revealed that very meager attention has been paid newlineon the sequential changes in the development of goat intestine during various stages of newlinegestation. Therefore, the present study was designed. The gross anatomical, histological and newlinehistochemical studies were conducted on the intestine of 36 goat embryos/ foeti of either sex newlinein different stages of gestation. The embryo/ foeti were grouped into I (0-50 days), II (51-100) newlineand III (101-till term). Different biometric parameters of each segment were recorded. Fixed newlinetissues from different segments were processed by routine paraffin embedding technique. 6 µ newlinethick paraffin sections were cut and stained by various staining procedure. Tissues fixed in newlinecold acetone were processed through acetone benzene schedule and then embedded in newlineparaffin of low melting point (48-500C) for localization of enzymes. Different histological newlineand histochemical observations were conducted in the stained slides under light microscope. newlineMicrometric observations were conducted for various parameters in each segment of intestine newlinein haematoxyline and eosin stained slides. All the segments of intestine were visible from 45 newlinedays of gestation onward. The jejunum was the longest segment and ileum was the shortest newlinesegment among all the segments of intestine. The diameter of rectum and jejunum was largest newlineand smallest, respectively among all the segments of intestine. Statistical analysis revealed newlinethat there was highly significant increase in all measured biometrical parameters of intestine newlinefrom group I to II and from group II to group III. Villi were appeared first time in newlineduodenum at 50 days of gestation followed by jejunum (51 days), while in other parts newlineit appeared at 58 days of gestation. Most of the villi were leaf/finger shaped in duodenum newlineand caecum, finger shaped in jejunum, club shaped in ileum and cone shaped in colon and newlinerectum. Surface epithelium was poorly differentiated stratified to pseudostratified columnar newlinetype in group-I (0-50 da