Evaluation of antidiabetic and antihyperlipidemic activities of marsdenia tenacissima and sphaeranthus indicus

Abstract

quotDiabetes diseases are characterized by irregularities in newlinecarbohydrate, protein, and lipid metabolism. These influence approximately 6 newlineto 10% of the population. Interest towards therapeutic agents derived from newlinenatural products is increasing throughout the world. Indigenous Plants are newlineused as source of medicine for treating diseases from ancient time. Now a newlinedays, plants are more commonly used as a origin of medicinal agents due to newlinetheir requisite properties. Different techniques (chemical/ biological) are used newlineto assess the important properties of medicinal plant extracts (1 4). newlineAim: The Aim of the present study is To Evaluate of antidiabetic and newlineantihyperlipidemic activities of Marsdenia tenacissima and Sphaeranthus newlineindicus. newlinePlan of work: newlineProcurement of Plants From market from reliable supplier newlineChemicals- Alloxan monohydrate from Sigma-Aldrich chemicals; Glucose, newlinetriglycerides, total cholesterol and HDL cholesterol kits from Span newlineDiagnostics, Gujarat. Parts of the plant used for the study: newlineMarsdenia tenacissima - Dried root newlineSphaeranthus indicus - Leaves, whole plant newlineThe whole study is divided in 6 phases-------- newlinePHASE I:- newline(i) Procurement and authentication of the plant material newline(ii) Preparation of various solvent extracts newline(iii) Preliminary phytochemical screening newlinePHASE II: In vitro antioxidant activity newlinePHASE III: Evaluation of different extracts for Anti-diabetic activity and newlineAntihyperlipdemic Effects. newlinePHASE IV: Evaluation for Antihyperlipidemic activity newline(i) Estimation of total cholesterol newline(ii) Estimation of HDL- cholesterol newline(iii) Estimation of triglyceride newline(iv) Estimation of LDL and VLDL-cholesterol newlinePHASE V: newlineComparative Studies on Antidiabetic effect with Biochemical and newlineHematological Parameters of Both Plants. newlinePHASE VI: newlineAn attempt has been made to evolve the comparative chemical profile of newlinethose fractions. Leaves of Sphaeranthus indicus and roots of newlineMarsdenia tenacissima were procured from the market and were identified newlinefrom Virani Science College, Rajkot; the voucher specimens (VSC/B- newline2/24/01/13 and VSC/B-3/1