Development and Validation of UV HPLC and HPTLC Analytical Methods for Quantification and Stability Studies of Newly Developed Antiviral Drugs Combination

Abstract

Pharmaceutical analysis usually involves experiments for the measurement of the newlineconcentration of drug as an active pharmaceutical ingredient (API) or component of a newlinepharmaceutical formulation. HPLC is a popular analytical technique and it is applied for newlineidentification, separation and quantification of each constituent of a mixture. Among the newlinevarious methods available for the determination of drugs, spectrophotometry continues to newlinebe very popular, because of their simplicity, specificity, and low cost. newlineIn this thesis work we have firstly done the literature survey and selected Ribavirin, newlineRitonavir and Lopinavir drugs for our study. We selected these three drugs because they newlinehave antiviral activity and may be effective in tackling the coronavirus disease 2019 newline(COVID-19) pandemic in combination. Few methods are available for simultaneous newlineestimation of these drugs but none of the reported analytical methods describe a method for newlinethe simultaneous determination of Ribavirin, Ritonavir and Lopinavir having less run time newlinewith good resolution. Hence, the present work was thought to develop a precise, accurate, newlinesimple and reliable, less time consuming method for simultaneous estimation of Ribavirin, newlineRitonavir and Lopinavir by HPLC, HPTLC and UV methods. newlineIn this UV Spectroscopy developed method we quantify the assay of Ribavirin, Ritonavir newlineand Lopinavir in formulations. Because Impurity of the Ribavirin, Ritonavir and Lopinavir newlineshow same lamada max of that main API, Hence this method also used for the newlineIdentification as well as dissolution purpose. The developed HPTLC method quantifies the newlineassay of the Ribavirin, Ritonavir and Lopinavir in formulatin. Because Impurity of the newlineRibavirin, Ritonavir and Lopinavir show same bands of that main API, Hence this method newlinexix newlinealso used for the Identification purpose. The developed HPLC method able to quantify the newlineimpurities of the Ribavirin, Ritonavir and Lopinavir in Pharmaceutical dosage form. newlineBecause Impurity of the Ribavirin, Ritonavir and Lopinavir show different peaks as newlinecompared w