Immobilized Lipase Catalyzed Biodiesel Production From Pongamia Seed Oil

Abstract

The environmental and economic concern regarding the use of fossil fuel at greater extent newlineencourages to find new alternative to fossil fuel that should be renewable, newlineecofriendly, economical and sustainable one, which could meet the growing energy newlinedemand. Biodiesel production via thermo chemical process have some issues related to newlineenvironment and cost of production, these issues can be answered with the use of enzymes. newlineIn the present studies, the lipase producing bacteria from local oil rich sample is isolated newlineand characterized by morphological and molecular studies using 16SrDNA analysis. The newlinespecies has been identified and confirmed as Lysinibacillus macroides FS1 (accession newlinenumber MW940857) and sequence is submitted to NCBI. For the same isolate, lipase newlineproduction media is optimized to enhance the lipase production. Optimized media results in newline5.4 fold higher production than innate media. During the present studies it is found that, newlinePongamia oil acts as inducer oil for the isolated strain. Further produced lipase is newlinecharacterized for methanol tolerance, pH stability and temperature stability. Immobilization newlineby entrapment method is optimized for the concentration of sodium alginate (4%) and newlineCaCl2 (200mM) to produce efficient immobilized beads. These immobilized beads which newlineare characterized for solvent stability and temperature stability under process operating newlineconditions reveals that they are suitable for biodiesel production. Km and Vmax values for newlinefree (Km 1.07mg/ml, Vmax 31.34U/ml) and immobilized lipase (Km 1.01mg/ml, Vmax newline27.02U/ml) are recorded. The produced biocatalyst is used for transesterification of newlinePongamia oil and reaction conditionsof transesterification is optimized through Response newlineSurface Methodology by Plackett- Burman and Box Behnken designs. Factors selected for newlinePlackett-Burman are, effect of molar ratio, amount of immobilized lipase (%), temperature newline(°C), reaction time (hour), pH, agitation speed (rpm) and water content (%) are studied. newlineAmong these seven variables amount of immob