Current progress in nanoparticulate drug delivery systems of m rna vaccines development and evaluation of a model peptide loaded plga based microsphere

Abstract

PLGA based microparticles have been investigated to a great extent for controlled drug newlinedelivery. It can be realized from the fact that, more than a dozen of these formulations newlinehave been commercialized for decades. Despite of their availability on market for years, newlinevery less literature is found on the optimization of process parametres in order to achieve newlinebetter control over burst release, in-vitro drug release, particle size distribution (PSD), newlinedrug and polymer distribution inside the microparticles and residual solvent etc. A newlinemicrosphere formulation is prepared by novel double emulsion solvent evaporation newlinetechnique. Preformulation study and characterization is performed. Trials are planned to newlineidentify the critical process parametres those impact the critical quality attributes of the newlinefinished microspheres. The process parametres are optimized to obtain microsphere of newlineright quality profile which release the GnRH agonist for a period of one month. Apart newlinefrom the above research work, the thesis includes a detailed review of Lipid nanoparticle newlinebased drug delivery systems including the mRNA delivery. The advantages of mRNA newlinevaccine technology are evaluated over the traditional platforms with example of m-RNA newlinedelivery COVID-19 vaccine. The thesis also demonstrates the importance of 3D printing newlinetechnology in managing the supply disruptions of medical devices and personal newlineprotective equipments (PPE) during COVID-19 pandemic. newline