Producation of Polyhydroxyalkanote PHA Utilizing Agro Industrial Waste

Abstract

Including soil from Parul university, cow manure, cow milk, pond water from Limda waghodia, tap water from Parul university garden water from the university s homoeopathic hospital and municipal water from Wghodia, Gujarat, a total of 18 bacterial stains were discovered. Eight of the 18 examined bacterial strains were able to accumulate PHA by responding favourably to Nile red staining. Four possible strains for PHA accumulation raged from 0.6-0.12% of cell dry weight, according to quantitative measurements of PHA from eight strains. A a carbon source, different vegetable peels were added to the medium in diverse proportions (2%, 4%, 6%, 8% and 10% w/v). According to the findings, the minimal salt media with Beetroot Peel Supplement (0.6%), Glucose Broth with Beetroot Peel Supplement (0.6%), and Glucose Broth with Pineapple Peel Supplement (0.4%) had the highest PHA yields. The Nutrient Broth with Beetroot Peel Supplement had the lowest PHA yields. A variety of sodium hypochlorite, sodium hypochlorite and chloroform, enzymatic digestion treatments, and chemical digestion of non-PHA cell material using sodium hydroxide and potassium hydroside. For the first time, Lysinibacilus fulsiformis, the highest PHA producer, has benn identified. Nile Red staining, PHA s solubility in various solvents, thermogravimetric analysis, gel permeation chromatography (GPS), H-NMR spectroscopy, FT-IR, and SEM were used to evaluate the extracted PHA. The average molecular wight (Mw) obtained was 52,826 and the polydispersity index was 32.8. The Mn value of the isolated PHA is 1608. newline